The degradation of visibility due to fogging on optical components, such as eyeglasses and safety goggles, in highly humid environments can pose serious safety risks. Passive anti-fogging methods, such as surface coatings are commonly employed, however, are easily damaged and have low durability. This paper presents a multiphysics approach to actively defog optical samples to enhance anti-fogging rates by ultrasonic methods. Tomographic measurements of micro-water droplets during exposure to ultrasonic loads are obtained by optical coherence tomography (OCT). A custom environmental chamber with integrated temperature and humidity controls was constructed. Ultrasonic loads are characterized using scanning laser Doppler vibrometer (SLDV) for frequencies up to 1 MHz. Multiple parameters, such as visibility, fog area, and dissipation rates, are monitored in real-time using a custom-developed program, and results are presented. Current work presents a promising solution for anti-fogging of optical substrates that have important applications across diverse industrial sectors.