Olive-growing has received particular interest in several regions of the world, including Brazil. There is a “new wave” of developments in this field, especially to improve at all stages of cultivation and processing the concepts of circular economy. This growth is also reflected in a crucial stage of the olive production chain: the nursery activity of seedlings. In the present research, Life Cycle Assessment (LCA) was used as a tool to identify the environmental impacts and establish prognostic scenarios in the context of clean technology in regard to two important aspects related to the production of seedlings in an industrial nursery in southern Brazil: the use of water for irrigation and the of packages for seedlings. The olive grove that is the focus of this research has a reference of four years of production of the Arbequina, Picual, Koroneiki and Frantoio varieties. It is observed that the orchard used as the basis of this study has characteristics representative of orchards in Rio Grande do Sul, falling within the classification of intensive cultivation, with high density without irrigation. In this study, the main hot spots for LCA were researched considering the recommendations of ISOs 14040 and 14044. The LCA methodology was applied using SimaPro 8.0.4 software, database Ecoinvent 3.1 and Recipe methods with Mid and Endpoints. Through analysis with Characterization, Normalization and Damage Assessment procedures, the main impacts were verified. The Scenario consisted of polyethylene pots with recycling and reuse of wastewater from the nurseries of the olive trees, and a functional unit considered as the production of 1000 units of seedling or 4 ha of planted area with 250 seedlings produced. Marine ecotoxicity was 0.84 kg 1,4-DBeq and freshwater ecotoxicity was 0.83 kg Peq. In regards to Damage Assessment, the impact on Human Health was 4.4 × 10–8 DALY and 1.72 × 10–10 species year−1 for Ecosystems. The impact on Resources was 2 × 10–3 $. With two scenarios proposed as Prognostics, the use of biocontainers reduces the impacts on Human Health and Ecosystems by approximately 80% and Resources by 100%, constituting a focus on innovation for the constant improvement of environmental management of olive trees nurseries. Constituting focus on innovation for the constant improvement of environmental management in the production of olive tree nurseries. As with other crops, the material balance for agricultural production includes, on the one hand, the introduction of nutrients necessary for production; and, on the other, the depletion of these by the harvesting of production. In the case of olive growing, around 85% of production turns into pomace from separation with great potential for replacing the nutrients needed for production. This aspect of environmental sustainability can be added to actions that have their carbon footprint and water footprint accounted for, using the LCA as a methodological reference.