Structural analysis is a fundamental aspect of civil engineering, focusing on understanding the behavior of structures under various loads and conditions. In this chapter, MATLAB is presented as a powerful computational tool that enhances the accuracy and efficiency of structural analysis tasks. The chapter covers a range of topics, starting from basic concepts such as internal forces, stresses, and deformations in beams, trusses, and frames, progressing to advanced methods like Finite Element Analysis (FEA) and dynamic simulations. With an emphasis on matrix methods, the chapter demonstrates how MATLAB can be used to model complex structures, solve large systems of equations, and perform detailed simulations to predict time-dependent behaviors like seismic responses. Finite element analysis, facilitated by MATLAB’s partial differential equation toolbox, allows for the modeling of complex geometries, boundary conditions, and stress distributions. Visualizations of structural responses, such as deformation shapes and stress maps, provide engineers with critical insights into the safety and performance of civil engineering structures. By integrating practical examples and case studies, the chapter guides readers through the process of applying MATLAB to real-world structural analysis problems, ensuring that they are equipped with the skills to handle both static and dynamic loading conditions in a professional context.