The usage of amniotic membranes is currently a hot topic in vitreoretinal surgery. The indications are a large macular hole, PVR perforations, optic disc pit and morning glory syndrome. We used an Optos photograph of the retina to determine the size of the amniotic membrane. The amniotic membrane consists of three layers; the epithelium, the stroma and the chorion. The latter has adhesive properties. Place the amniotic membrane with the sticky side (chorionic side) on the retina. The amniotic membrane is best fixated under the retina in order to prevent future dislocation. An epiretinal placement is necessary in pathologies27G TDC cutterpathologies such as optic disc pit or morning glory syndrome (Figs. 16.1, 16.2, 16.3, and 16.4). For epiretinal amniotic membranes a silicone oil tamponade is advisable because gas tamponades result often in dislocations.