The increasing interest in foods that can have a positive influence on the health of human is highlighting certain goods that have been traditionally utilized within local communities for generations, but have recently garnered global recognition. Current research suggests that pumpkin seed oil, that has long been utilized in traditional medicine as well as cooking, may potentially find application in the cosmetic and pharmaceutical industries. Furthermore, certain sources classify it as a prospective functional food due to its derivation from pumpkin seeds, which are rich in various functional constituents. Nevertheless, the oil’s production procedure might impact the composition of these elements, hence influencing the oil’s biological activity. The purpose of this review is to offer a brief summary of the scientific literature examining potential of pumpkin seed oil (PSO) as a functional food ingredient. This chapter provide an extensive analysis of the safety, biological activity, phytochemical content, as well as chemical composition of PSO. The primary bioactive compounds found in pumpkin seed oil that have health-promoting effects include polyphenols, phytoestrogens, and fatty acids. Furthermore, tocopherols, minerals, carotenoids, and squalene have the potential to contribute to the diverse benefits of health associated to this oil. The majority of investigations have been carried out in a controlled laboratory environment and provide evidence to support the assertion that PSO possesses antioxidant as well as antibacterial properties. Empirical research has demonstrated that PSO may have advantageous effects in addressing cardiovascular issues in menopausal women and conditions linked to an irregularity in sex hormones.