Despite the potentialities in the early diagnosis of oncologic diseases, the analysis of the volatile component of breath, urine, and feces is far from being routinely implemented in clinical practice due to the lack of sampling standardized procedures able to guarantee a good repeatability and comparability of results. The methodological approach based on the collection of samples inside containers of different typology (polymeric bags, stainless-steel canisters) has been over the decades the most applied within the international scientific community. However, in order to overcome several limitations, mainly related to the representativeness of the volatile fraction and to the potential sample contamination over multiple analytical steps, in particular for breath, automated sampling devices designed to collect volatile organic compounds (VOCs) in human breath directly onto sorbent cartridges have been recently developed and validated. Scientific research and technological advancement towards standardization of the sampling techniques are currently being promoted within the international research community to achieve harmonization and increase robustness of experimental data worldwide.