The main environmental problems of paramount importance are the increased volume of waste produced, accumulation and disposal of toxic garbage, and climate change due to greenhouse gas emissions into the atmosphere, particularly carbon dioxide. Russia has a status of the “major economy,” which is focused on extractive industries and natural resources. As a result, the country has many problematic issues in the field of environmental pollution, namely the negative impact of regional enterprises and hazardous waste management. In this regard, the research explores the scientific category “ecological portrait of a region” against the background of the ESG concept. The research considers the national project “Ecology” and the ESG concept from the perspective of a regional ecological portrait establishment. The authors determine the essence of the term “ecological portrait of a region” and the stages of drawing up an ecological portrait of the region. Moreover, the authors present indicators for each stage that can be used to assess the environmental safety, management, and efficiency of the region. Additionally, they describe the relevant aspects of a regional ecological portrait. The research results allowed the authors to conclude that eco-efficiency is an express indicator of a regional ecological portrait.