Nanomaterials bear promises for a range of agricultural applications, such as environmental remediation, improving stress tolerance, and delivering nutrients to crop plants. Although they enhance the effectiveness of agricultural chemical, their addition could pose risks to food supply, environment, and human health. Therefore, monitoring nanoparticles’ uptake, transport, conversion, interaction with higher plants, and potential hazards in agriculture is crucial. To maximize the positive effects of nanomaterials while minimizing their ecological consequences, it is imperative to comprehend the complex methods by which plants interact with them. This book chapter gives a thorough review of the interactions that occur between plants and nanomaterials, with special focus on the absorption, transport, and accumulation processes. It explores the various routes that plants use to absorb nanomaterials, including seed priming, foliar application, and root uptake. Moreover, the chapter provides insight into the mechanisms of nanomaterial translocation and transport throughout plant tissues. The consequences of nanomaterial accumulation in edible plant parts are also covered in this chapter, emphasizing the significance of determining the safety of nanomaterials and their bioavailability for human ingestion in nutrient-loaded nanomaterials. Furthermore, it offers insights into the intricate interactions between nanomaterials and plants by reviewing the present knowledge and research gaps. This lays the groundwork for future investigations into the topic of nanotechnology for environmental stewardship and sustainable agriculture.