Maintaining specimen orientation during tissue transportTissue transport and processing is paramount for the success of Mohs micrographic surgeryMohs micrographic surgery(MMS)Mohs micrographic surgery initial processing. Once the specimen is removed from the surgical site and hemostasis is achieved, a Mohs mapMohs map should be immediately created and correctly oriented. The removed specimen is then transported to the histopathologyHistopathologylaboratoryLaboratory for further processing. The specimen should be cut into as few sections as possible to avoid orientation errors, while still producing high-quality slides. Colored ink is used to assist in maintaining orientation, while optimum cutting temperature (OCT) is used to facilitate specimen adhesion to the microtome chuck. Following microtome sectioning, the specimen is stained, most commonly with hematoxylin and eosinHematoxylin and eosin (H&E). After application of the coverslip, the slides are ready to be analyzed by the MohsMohs surgeon.