The paper presents an analysis of the effect of regular and irregular segmentations on the characteristics and charge distribution densities of microstrip lines (MSLs) with three strip cross-sectional shapes (rectangular, rounded at the top, and fully rounded). The analysis is based on the results obtained in the TALGAT quasi-static electromagnetic compatibility simulation system. The results demonstrate that segmentation can affect the construction of the shapes of conductors when they are rounded but not rectangular. At the same time, a change in segmentation can affect the charge distribution density and characteristics of all conductor shapes. Meanwhile, to build a real shape of a rounded conductor, it is necessary to reduce the length of segments along the entire length of the structure with regular segmentation. With irregular segmentation, the length of segments should be reduced only at the edges of the conductor, while leaving a rough segmentation value along the rest of the width. In this regard, irregular segmentation is the most advantageous as it reduces computational time and memory costs.