Nanotechnology has transformed numerous industries, particularly food packaging, by providing creative solutions to increase food safety, shelf life, and overall quality. Nanomaterials, which have sizes ranging from 1 to 100 nanometers, exhibit distinctive characteristics such as increased surface area, improved barrier performance, and superior mechanical strength, rendering them highly desirable to be employed in food packaging. The use of nanoparticles in packaging provides several advantages, such as extending the shelf life of food by creating barriers that block the entry of moisture, gasses, and pollutants, so preserving its freshness and quality. Nanocomposites enhance the mechanical properties of packaging materials, hence minimizing the risk of damage during the process of handling and transportation. The use of nanosensors into packaging allows for the creation of intelligent systems that can continuously check the quality, freshness, and safety of food, hence increasing consumer confidence. Nanotechnology enables the development of sustainable packaging solutions, which help protect the environment by decreasing waste and minimizing environmental effects. Nevertheless, the increasing utilization of nanoparticles in food packaging highlights the immediate requirement for accurate detection and analysis methods to guarantee safety and adherence to regulations. Precise detection techniques are essential for monitoring the presence of nanoparticles, evaluating the potential dangers to human health, and assuring compliance with regulatory criteria. Moreover, providing clear and unambiguous information regarding nanoparticles enhances consumer trust and facilitates well-informed decision-making. Advanced analysis techniques are crucial in research and development for analyzing the behaviour of nanomaterials and consistently enhancing safety protocols. This chapter provides a thorough examination of detection approaches and analytical techniques used to identify nanoparticles in food packaging. It covers topics such as regulatory frameworks, safety considerations, quantification, characterization, case studies, and future research prospects.