Cuticle constitutes the outermost layer of insect’s body. It is composed of three distinct layers: outer envelope, epicuticle, and procuticle. The main chemical compositions of the cuticle include chitin, proteins, and lipids. The insect growth is limited by the cuticle, so it must be shed and replaced, which is called molting. Locusta migratoria is a typical hemimetabolous insect species. It has a three-stage life cycle, including egg, five nymph instars, and adult. During its development, locust must molt from one stage to another, which involves the chitin, protein and lipid synthesis and metabolisms. As a result of technological advances in omics and molecular biology, including genomics, proteomics and RNAi, a wealth of new information on these processes has been uncovered, particularly in the identification and characterization of the genes and proteins involved in biosynthesis and degradation of chitin, interactions between chitin and cuticular proteins, and biosynthesis and transport of cuticular lipids. In this chapter, we describe various genes and proteins and their functions involved in chitin metabolism lipid metabolism, and cuticular formations in L. migratoria. These genes could be selected as potential target genes for RNAi-mediated pest management.