This chapter of Women Scientists in American Television Comedy: Beakers, Big Bangs and Broken Hearts reviews existing literature examining the persistent underrepresentation of women in STEM fields and how television portrayals contribute to this disparity. We highlight how cultural stereotypes present science as a masculine pursuit, where women scientists are frequently depicted through limiting narratives that prioritize traditional gender roles, personal conflicts and diminished competence. Such portrayals can influence public perceptions and discourage young women from pursuing STEM careers. The chapter delves into the impact of humor as both a reinforcing and challenging force in these portrayals. By framing women scientists as sources of comic relief, TV shows risk trivializing their professional identities, which can perpetuate stereotypes. However, the chapter also explores how comedy has the potential to disrupt these norms, offering viewers alternative, empowering representations of women in STEM. An intersectional perspective is considered in how media portrayals affect not only women but also marginalized groups in STEM. We address how the use of ironic and feminist humor in popular media can activate audience reflection on gender norms, supporting broader engagement and cultural change. Through an examination of humor’s role in representation, this chapter sheds light on the complexities of gender, STEM and media influence, underscoring humor’s power to challenge stereotypes and foster positive shifts in how women scientists are perceived in American television.