Nowadays, there are plenty of microfluidic-based devices, from microfabrication to micro total analysis systems (microTAS) and lab-on-a-chip (LOC). The applications of these devices are very vast, such as inkjet printers, biomedical, environmental, food, smart textiles, and the detection of diseases. Microdevices mainly contain microchannels, micropumps, chambers, and membranes, and the processes are based on fluid dynamic principles. However, there are many challenges in the acceleration of these techniques. Therefore, this research investigates the main flow properties, methods, equations, boundary conditions, control points, and software applications in simulating the microfluidic behaviors in the sensors and microdevices. In this regard, after an intensive literature review analysis, hemodynamic behavior in a microdevice glucose kit has been investigated through experimental tests and CFD simulation. The results show good agreement among CFD simulation and experimental data.