Aim: To assess the sonolytic breakdown of polyquaterium in artificial aqueous media under controlled conditions. Materials and methods: Distilled water and polyquaterium were combined to create a chemical solution that ranges in pH from 4.4 to 7. Results were recorded for each of five different time intervals, and pH values between 4.5 and 8.5 were listed in a table for each interval of ten minutes. Group 1 and group 2 of each five samples were taken for each pH solution, and a one-way Anova test was performed using IBM SPSS Version 26 to compare the samples using a numerical type of analysis with the confidence interval 95%, G-power 80%, and enrollment ratio all set to one (N = 10). Results: Freshly made polyquaterium stock solution was examined for ideal temperature and pH range. The results were examined and recorded. The initial concentration of the sample solution was set at 100 mg/L, and a pH range of 4.5 to 8.5 was taken into account. The solution’s absorbance at 540 nm wavelength was measured using a UV–Vis spectrophotometer. For statistical observation, the mean–variance was compared between Group 1 and Group 2 using a one way ANOVA test that reveals the significance value p = 0.001 (p < 0.05). Conclusion: The effectiveness of removal and the frequency of response during the sonolysis process may both be impacted by temperature. A pH and temperature were indeed two of the factors that affect how effectively polyquaterium can be taken down by sonolysis. For the breakdown of polyquaterium sonolysis operates efficiently.