This chapter discusses the role of biomechanics in regenerating bone and musculoskeletal tissues, emphasising the incorporation of mechanical characteristics into tissue-engineered scaffolds. The unique structure of bone is described, highlighting its viscoelastic and other properties crucial for tissue regeneration and scaffold development. Developments in scaffold materials, including natural, synthetic and composite varieties, and the significance of mechanotransduction in tissue integration and engineering are discussed. In addition, the biomechanical aspects of cartilage, tendon, and muscle are discussed, reflecting their importance in musculoskeletal repair Emerging technologies that accurately replicate the mechanical characteristics are also discussed, with a focus on their potential to enhance outcomes in regenerative medicine.