Many techniques of soil remediation are available but the use of enzyme technology and microbial bioprocesses is eco-friendly and cost effective due to the less energy input requirements. Enzymes especially the oxidoreductases are highly valuable for the removal/degradation of toxic contaminants (polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCBs)) from the soil. Understanding about the mode of action of various biocatalysts and their optimum conditions are well documented, but still their full implementation at a commercial scale is awaited due to their less open environmental stability and cost intensive continuous accessibility. Persistent pollutants from the soil should be eliminated through applying the potent active bio-enzymes that is possible through in situ or ex sit strategies. In this chapter, authors critically focused on the various biocatalysts that are helpful for the bioremediation of polluted soil.