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For over 20 years, DNA barcoding has participated in characterizing the molecular diversity of wild bees worldwide and rapidly advancing our taxonomic knowledge of this group of pollinators. This chapter first presents the background of the emergence of DNA barcoding for the study of wild bees and its large contributions to bee taxonomy through revision, the clarification of historically problematic groups and species discovery. The second part outlines the long-step methodological process required for obtaining high-quality and informative reference barcodes. This section also provides some recommendations for the construction of DNA barcode libraries. Eventually, this chapter presents how DNA-based approaches (barcoding, metabarcoding and environmental DNA) would be of great interest for large-scale and long-term routines of bee monitoring.

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Molecular Identification of Wild Bees

  • 章节

  • 发表日期：

    2025年

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  • Melodie Ollivier,
  • Giovanni Cilia,
  • Diego Cejas

摘要

For over 20 years, DNA barcoding has participated in characterizing the molecular diversity of wild bees worldwide and rapidly advancing our taxonomic knowledge of this group of pollinators. This chapter first presents the background of the emergence of DNA barcoding for the study of wild bees and its large contributions to bee taxonomy through revision, the clarification of historically problematic groups and species discovery. The second part outlines the long-step methodological process required for obtaining high-quality and informative reference barcodes. This section also provides some recommendations for the construction of DNA barcode libraries. Eventually, this chapter presents how DNA-based approaches (barcoding, metabarcoding and environmental DNA) would be of great interest for large-scale and long-term routines of bee monitoring.