The study aimed to determine the relationship effect of work quality between human capital and organizational performance in private higher educational institutions. The analyitical descriptive, quantitative method was used. The study population consisted of (8) private higher educational institutions, and for collecting data, a comprehensive survey method was used, which was limited to all directors, assistance, and heads of departments with a total number was (477) respondents. The software of (SPSS- V20) was used for data statistical analysis of the study hypotheses tests, and (AMOS –V24) path analysis was used for the mediation role. The study findings showed that there is statistically significant impact of the human capital on organizational performance at the significance level (α ≤ 0.05), and there is a partial relationship effect of work quality as an mediate variable between human capital and organizational performance at the significance level (α ≤ 0.05), and the most contributions of the study is private higher educational institutions administrations requires to pay more attention to employees functional and moral ensure a positive organizational performance by qualified and skilled, decision making participation, experience, creativeness and innovative ideas to enhance organizational performance and work quality,and to pay more attention to human capital as one of the important intellectual assets for a better future to enhance the overall job performance. The study also recommends future studies with other dimensions and environments of human capital on organizational performance. The study contributes aims to assist organizations management to improve organizational performance and work quality.