PMMA is used extensively for fabricating dentures due to its compatibility, processing ease, and esthetics. However, its low strength often results in frequent fractures,, which are repaired using autopolymerizing resins that retain 60–65% strength. This study aim to evaluate the flexural strength of autopolymerizing resin with various concentrations of biosynthesized rGO NPs and compare the strength difference between reinforced and unreinforced forms. 64 Specimens were used for testing.. Groups A, B, and C were reinforced with 0.1, 0.05, and 0.025% reduced Graphenes Oxide (rGO) nanoparticleswith group D as Control. All the groups were tested for flexural strength and the data was analyzed by SPSS Version 25 software. Incorporation of 0.1% rGO into autopolymerizing resin enhanced flexural strength by 2.69 times. On the contrary, there was no significant difference in the mean values obtained between 0.025% rGO and 0.05% rGO incorporated resin.