This chapter describes specific techniques plus general curricular approaches to performing CRISPR/Cas9 genome editing in Drosophila in a classroom environment. We present several different scenarios for performing CRISPR in Drosophila, that can be adapted depending upon the length of time available for the class, and the technology available to the instructor and students.

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CRISPR in Drosophila

  • Kumar Vishal,
  • Jeffrey L. Van Zant,
  • Richard M. Cripps

摘要

This chapter describes specific techniques plus general curricular approaches to performing CRISPR/Cas9 genome editing in Drosophila in a classroom environment. We present several different scenarios for performing CRISPR in Drosophila, that can be adapted depending upon the length of time available for the class, and the technology available to the instructor and students.