This study builds upon existing odometry calibration formulation for two-wheeled differential drive robots. The proposed approach increases the potential for autonomous calibration by utilising closed-loop control where the mobile robot is programmed to follow a line marked out on a surface. The proposed approach negates the need for external measurements made by a human operator within the calibration process. This study makes a comparative analysis and evaluation between the presented approach against an established prior approach by implementing both on a real (not simulated) mobile robot and capturing performance data.