In the present study, we aim to demonstrate the production of bioelectricity using the EUOVh_ID3 strain previously isolated from an electroactive biofilm. This strain was previously identified as Enterobacter sp. The study was carried out using two electrochemical methods, including imposed potential chronoamperometry (IPC) under +0.4 V/SCE potential and cyclic voltammetry (CV) at a scan rate of 10 mV/s. The experiments were carried out in a three electrode electrochemical device, supplied with glucose as the sole carbon source, without the addition of any external mediator. When the electrochemical behavior of strain EUOVh_ID3 was demonstrated, a maximum current density of 13 µA/cm2 was recorded on 7th day of the IPC. The oxidation peaks recorded during cyclic voltammetry suggested that the electrochemical activity of strain EUOVh_ID3 could be linked to membrane proteins such as c-type cytochrome or the pili conductor, which could be used for direct electron transfer between the bacteria and the working electrode.