This chapter discusses the concept of One-Piece Flow (OPF), its origins, scope, main critical success factors, and barriers. Also reports a small bibliometric analysis of OPF and Manufacturing and findings indicate that the United States of America is the country with the highest academic production on these topics, Lander, and Liker were the authors with the highest number of citations (150) and the International Journal of Production Research was the leading journal. In addition, it includes an application case where the material flow is shown at the implementation time in the production process of a packaging company working with a traditional batch system, and the fundamentals of OPF are implemented. The results show that OPF reduces delivery times by at least 34%, eliminating unnecessary transportation and activities that do not add value to the process, such as unnecessary product inspections.