Microplastic distribution and its several effects are some of the most challenging problems of humanity on these days and, due to the enormous plastic debris quantity in every ecosystem it’s imperative to find new solutions. On this research, it has been proved a method to isolate and identify microplastic in macroinvertebrate, besides, distribution of microplastics was determined for the first time in Camana river. The method consisted in sampling with surber nets of 500 µm, that is a usual method in macroinvertebrates sampling. Microplastic isolation was performed first by an organic matter digestion with peroxide at 35% for 7 days. The microplastics obtained were analyzed by a µFTIR obtaining the chemical structure and particles quantification by the OMNIC Picta software. It’s been found polyethylene, polyester, silicon polymer and silicon polymer into macroinvertebrates. To determine the sensitiveness of the macroinvertebrates was necessary to identify and apply the diversity indexes showing dominance in the sampling point 3 owing to the poor quality of water sampled, besides the indicator value showed Elmidae family as a potential environmental indicator of microplastic due to its sensitiveness of this pollutant. Finally, microplastic abundance was not relevant to macroinvertebrates diversity at this period and at this location, however it was possible for the first time to evidence the microplastic presence in macroinvertebrates in Camana river that was validated and revealed by infrared techniques.