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To monitor the kinetics of enzymatic processes, a whole cascade of physicochemical methods is used. This chapter is devoted to a brief review of the physical foundations of these methods, the application of which is illustrated by typical examples. The most traditional and widely used methods are conventional absorption, infrared, Raman and luminescence spectroscopies with timescales from 10−14 s to 10−2 s. Much less commonly used are such developed physical methods as dielectric relaxation (DR), depolarized light scattering (DLS), optical Kerr-effect (OKE), ultrasound, terahertz (THz) spectroscopies, femtosecond pump–probe, photon-echo, optical Kerr effect, sum-frequency generation, two-dimensional infrared spectroscopic two-dimensional sum-frequency generation, three-dimensional infrared, and two-dimensional Raman terahertz spectroscopy.

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Methods of Analysis in Enzyme Processes

  • 章节

  • 发表日期：

    2025年

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  • Gertz I. Likhtenshtein

摘要

To monitor the kinetics of enzymatic processes, a whole cascade of physicochemical methods is used. This chapter is devoted to a brief review of the physical foundations of these methods, the application of which is illustrated by typical examples. The most traditional and widely used methods are conventional absorption, infrared, Raman and luminescence spectroscopies with timescales from 10−14 s to 10−2 s. Much less commonly used are such developed physical methods as dielectric relaxation (DR), depolarized light scattering (DLS), optical Kerr-effect (OKE), ultrasound, terahertz (THz) spectroscopies, femtosecond pump–probe, photon-echo, optical Kerr effect, sum-frequency generation, two-dimensional infrared spectroscopic two-dimensional sum-frequency generation, three-dimensional infrared, and two-dimensional Raman terahertz spectroscopy.