The 19-item Fears and Resistances to Mindfulness (FRM) scale is a self-report instrument for measuring difficulties engaging with mindfulness practices. Exploratory and confirmatory factor analyses supported a two-factor structure, “fears” and “resistances,” which showed good fit to the data. Moderate to strong correlations were found between fears of mindfulness and depression, anxiety, stress, self-criticism, and fears of compassion, while resistances to mindfulness also significantly correlated with these variables to a lesser degree. The scales demonstrated excellent internal reliability, good test-retest reliability, and concurrent validity with related concepts. Data from a Turkish adaptation was largely consistent with original findings. Fears of mindfulness also correlated negatively with life satisfaction and mindfulness, further indicating divergent validity. The FRM scale may be useful for identifying individuals who are likely to struggle with mindfulness and provide insight into specific difficulties, which may be amenable to psychotherapeutic and self-help interventions.