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To address the limited understanding of polysaccharide biosynthesis in medicinal macrofungi, we present an integrated multi-omics protocol. Our method details the entire workflow, from experimental design and de novo genome assembly to transcriptomic and metabolomic profiling. Key genes are pinpointed through weighted gene co-expression network analysis and KEGG pathway mapping, followed by experimental validation using RT-qPCR. This established pipeline provides a robust framework for elucidating biosynthetic pathways and guiding strain improvement.

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Omics-Based Insights into Polysaccharide Biosynthesis in Macrofungi

  • 实验方案

  • 发表日期：

    2026年

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  • Xing Luo,
  • Ji-Hang Jiang,
  • Li-Wei Zhou

摘要

To address the limited understanding of polysaccharide biosynthesis in medicinal macrofungi, we present an integrated multi-omics protocol. Our method details the entire workflow, from experimental design and de novo genome assembly to transcriptomic and metabolomic profiling. Key genes are pinpointed through weighted gene co-expression network analysis and KEGG pathway mapping, followed by experimental validation using RT-qPCR. This established pipeline provides a robust framework for elucidating biosynthetic pathways and guiding strain improvement.