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In situ hybridization (ISH), fluorescent in situ hybridization (FISH), and the most advanced RNA in situ hybridization methodology termed RNAscope® enable us to detect the expression and localization of a specific RNA in fixed cells or tissue sections. Here, we describe in detail three procedures adjusted to reveal specifically noncoding RNAs (ncRNAs) in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the three different approaches are also shown.

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Identification of Long Noncoding RNA by In Situ Hybridization Approaches

  • 实验方案

  • 发表日期：

    2026年

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  • Mara Mancini,
  • Anna Maria Lena,
  • Eleonora Candi

摘要

In situ hybridization (ISH), fluorescent in situ hybridization (FISH), and the most advanced RNA in situ hybridization methodology termed RNAscope® enable us to detect the expression and localization of a specific RNA in fixed cells or tissue sections. Here, we describe in detail three procedures adjusted to reveal specifically noncoding RNAs (ncRNAs) in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the three different approaches are also shown.