The combination of optogenetic manipulations and neural recordings is a powerful approach to studying brain processes associated with specific neural circuits. The link between neural circuits and overall brain function further relies on comprehensive datasets that consist of both behavioral observations and large-scale neural recordings. In particular, functional ultrasound (fUS) and functional MRI (fMRI) are brain-wide imaging modalities that can be performed in rodents alongside optogenetic perturbations. These modalities record similar hemodynamic signals and, at the same time, complement one another by enabling neural recordings under different behavioral contexts. Despite the similarities between these modalities and their increasing utility with neural circuit investigations, multimodal experiments are far and few between. This chapter focuses on how optogenetics can be combined with both fUS imaging and fMRI in rodents, highlighting key experimental considerations, surgical preparation, and timelines that optimize data quality.