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Laser microdissection (LMD) enables the precise isolation of specific cells or regions of interest from tissue sections, guiding downstream molecular analyses with high spatial resolution. In this protocol, we describe an optimized protocol combining whole-slide immunofluorescence imaging, LMD, and low-input liquid chromatography (LC) mass spectrometry (MS)-based proteomics of FFPE tissue sections.

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Immunofluorescence Imaging-Guided Laser Microdissection for Ultralow Input Spatial Tissue Proteomics

  • 实验方案

  • 发表日期：

    2026年

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  • Sonja Fritzsche,
  • Fabian Coscia

摘要

Laser microdissection (LMD) enables the precise isolation of specific cells or regions of interest from tissue sections, guiding downstream molecular analyses with high spatial resolution. In this protocol, we describe an optimized protocol combining whole-slide immunofluorescence imaging, LMD, and low-input liquid chromatography (LC) mass spectrometry (MS)-based proteomics of FFPE tissue sections.