Scholarlink

In addition to the well-known applications of bacterial artificial chromosomes (BACs) in classical molecular genetics, these probes are also well suited for molecular cytogenetic studies. BACs are currently the most commonly used locus-specific probes in FISH. Various applications are possible, such as gene mapping, FISH banding, determination of chromosome breakpoints, characterization of derived chromosomes, investigations of interphase architecture, or karyotype evolution studies. Here, the basic principle of how BACs can be hybridized in situ on chromosome preparations is described. In addition, an overview of possible questions that can be investigated with BACs as FISH probes is provided.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Homemade Locus-Specific FISH Probes: Bacterial Artificial Chromosomes

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Thomas Liehr,
  • Stefanie Kankel

摘要

In addition to the well-known applications of bacterial artificial chromosomes (BACs) in classical molecular genetics, these probes are also well suited for molecular cytogenetic studies. BACs are currently the most commonly used locus-specific probes in FISH. Various applications are possible, such as gene mapping, FISH banding, determination of chromosome breakpoints, characterization of derived chromosomes, investigations of interphase architecture, or karyotype evolution studies. Here, the basic principle of how BACs can be hybridized in situ on chromosome preparations is described. In addition, an overview of possible questions that can be investigated with BACs as FISH probes is provided.