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We describe how to perform simultaneous immunodetection using fluorescence and RNA-fluorescence in situ hybridization (immuno-RNA-FISH). The aim of the approach is to localize proteins involved in epigenetic regulation (histone modifications) using immunodetection and the mRNA of genes of interest using RNA-FISH. This article describes an optimized protocol for mouse embryos that has been adapted for this specific biological material to preserve the three-dimensional (3D) organization of cell nuclei.

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Three-Dimensional Co-immunodetection and RNA-Fluorescence In Situ Hybridization in Preimplantation Mouse Embryos

  • 实验方案

  • 发表日期：

    2026年

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  • Martine Letheule,
  • Amélie Bonnet-Garnier

摘要

We describe how to perform simultaneous immunodetection using fluorescence and RNA-fluorescence in situ hybridization (immuno-RNA-FISH). The aim of the approach is to localize proteins involved in epigenetic regulation (histone modifications) using immunodetection and the mRNA of genes of interest using RNA-FISH. This article describes an optimized protocol for mouse embryos that has been adapted for this specific biological material to preserve the three-dimensional (3D) organization of cell nuclei.