The DUX (Double homeobox) gene family plays a crucial role during early development, particularly in the activation of the embryonic genome. The higher organization of chromatin, as well as the spatial localization of genes in the nucleus and their proximity to heterochromatin, may play a role in regulating their transcriptional activity. Fluorescence in situ hybridization (DNA-FISH) is a widely used technique for accurately locating and visualizing specific DNA sequences in their chromatin context. In this methodological article, we describe the application of the DNA-FISH technique to the study of the DUX gene in bovine embryos. The protocol includes the design and synthesis of specific DNA probes, embryo preparation, and detailed steps for hybridization and imaging. We also address the challenges and optimizations required for studying the DUX gene in bovine embryo models. This detailed method allows us to better understand the genomic positioning of the gene and, consequently, the presumed regulatory mechanisms, thus contributing to the broader field of genetic and epigenetic regulation in early development. This method serves as a practical guide for researchers studying the molecular basis of embryonic development in bovine models, offering insights into the technical and biological aspects of using the DNA-FISH technique for gene localization studies.