Studies of chromosomes at the stage of meiotic prophase I, specifically synaptonemal complexes (SC), have recently become an important part of many works on cytogenomics. The larger physical size of pachytene bivalents, in contrast to metaphase mitotic chromosomes, allows for the effective use of light fluorescence microscopy, immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). An important feature of spread SCs is the preservation of not only DNA but also many proteins associated with meiosis. Therefore, the study of SCs provides data on complex and multi-stage transformations of chromatin during this key stage—prophase I of meiosis. This work provides information on the specifics of the surface-spreading technique of meiotic cells, ICC, and FISH.