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Multicolor fluorescence in situ hybridization (mFISH) assays are indispensable for a precise description of complex chromosomal rearrangements and marker chromosomes. Routine application of such techniques on human chromosomes began in 1996 with the simultaneous use of all 24 human whole chromosome painting (wcp) probes in multiplex-FISH (M-FISH) and spectral karyotyping (SKY). Here, we present an overview of the available mFISH approaches using wcp probes and describe a basic protocol for M-FISH and SKY.

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Multiplex FISH and Spectral Karyotyping

  • 实验方案

  • 发表日期：

    2026年

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  • Thomas Liehr

摘要

Multicolor fluorescence in situ hybridization (mFISH) assays are indispensable for a precise description of complex chromosomal rearrangements and marker chromosomes. Routine application of such techniques on human chromosomes began in 1996 with the simultaneous use of all 24 human whole chromosome painting (wcp) probes in multiplex-FISH (M-FISH) and spectral karyotyping (SKY). Here, we present an overview of the available mFISH approaches using wcp probes and describe a basic protocol for M-FISH and SKY.