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Blood smears, bone marrow smears, and effusions can be very helpful for rapid molecular cytogenetic diagnostics in leukemia, lymphomas, or even solid tumors. Such samples can be examined with suitable interphase FISH probes or probe sets for diagnostics, prognostics, therapeutic decisions, follow-up care, and monitoring the effects of medications or the success of a bone marrow transplant. Here you will find two protocols for preparing smears or cell suspensions in methanol/acetic acid (3:1) for subsequent FISH analysis.

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Tumorcytogenetic Diagnostics and Research on Blood and Bone Marrow Smears or Effusions

  • 实验方案

  • 发表日期：

    2026年

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  • Anita Glaser,
  • Thomas Liehr

摘要

Blood smears, bone marrow smears, and effusions can be very helpful for rapid molecular cytogenetic diagnostics in leukemia, lymphomas, or even solid tumors. Such samples can be examined with suitable interphase FISH probes or probe sets for diagnostics, prognostics, therapeutic decisions, follow-up care, and monitoring the effects of medications or the success of a bone marrow transplant. Here you will find two protocols for preparing smears or cell suspensions in methanol/acetic acid (3:1) for subsequent FISH analysis.