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Molecular cytogenetic approaches applying smaller, locus-specific probes such as cDNA, plasmids, cosmids, fosmids, P1-clones, bacterial artificial chromosomes (BACs), or yeast artificial chromosomes (YACs) can sometimes be compromised by inefficient hybridization. In addition, FISH results may be required within a few hours, particularly in diagnostics. Here, we present a FISH protocol using microwave treatment that results in better hybridization efficiency with smaller probes and evaluable results within a few hours.

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Microwave Treatment for Better FISH Results in a Shorter Time

  • 实验方案

  • 发表日期：

    2026年

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  • Anja Weise,
  • Thomas Liehr

摘要

Molecular cytogenetic approaches applying smaller, locus-specific probes such as cDNA, plasmids, cosmids, fosmids, P1-clones, bacterial artificial chromosomes (BACs), or yeast artificial chromosomes (YACs) can sometimes be compromised by inefficient hybridization. In addition, FISH results may be required within a few hours, particularly in diagnostics. Here, we present a FISH protocol using microwave treatment that results in better hybridization efficiency with smaller probes and evaluable results within a few hours.