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This chapter presents a comprehensive set of protocols for isolating chromatin and histones from Trypanosoma cruzi, tailored for applications ranging from gel-based analyses to high-resolution mass spectrometry. It outlines optimized workflows for extracting basic nuclear proteins, histones, linker histone H1, and chromatin-associated proteins, adapted to different parasite life stages and cell cycle phases. By integrating multiple approaches, these methods address challenges posed by the parasite’s unique biology and provide flexible tools for studying histone post-translational modifications and chromatin proteins. The inclusion of protocols compatible with proteomic workflows supports broader investigations into epigenetic regulation and nuclear processes in T. cruzi.

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Multi-Approach Workflows for Chromatin and Histone Isolation from Trypanosoma cruzi: From Basic Protein Extraction to Mass Spectrometry-Compatible Protocols

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  • 发表日期：

    2026年

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  • Ana Paula Menezes,
  • Julia Pinheiro Chagas da Cunha

摘要

This chapter presents a comprehensive set of protocols for isolating chromatin and histones from Trypanosoma cruzi, tailored for applications ranging from gel-based analyses to high-resolution mass spectrometry. It outlines optimized workflows for extracting basic nuclear proteins, histones, linker histone H1, and chromatin-associated proteins, adapted to different parasite life stages and cell cycle phases. By integrating multiple approaches, these methods address challenges posed by the parasite’s unique biology and provide flexible tools for studying histone post-translational modifications and chromatin proteins. The inclusion of protocols compatible with proteomic workflows supports broader investigations into epigenetic regulation and nuclear processes in T. cruzi.