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Monoxenous trypanosomatids exclusively parasitize insects and are powerful systems for exploring trypanosomatid biology, the evolution of parasitism, and host–parasite interactions. In addition, they are tractable early-diverging eukaryotes that can serve as model organisms for a variety of conserved processes. We have elaborated on existing methods for culturing the mosquito parasite Crithidia fasciculata and the bumblebee parasite Crithidia bombi, including quantitative assessment of surface attachment and genetic modification. In addition, as cultured strains retain their ability to infect their insect hosts, we describe use of a luciferase-based reporter line to quantitate infection intensities in laboratory mosquitoes.

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Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi

  • 实验方案

  • 发表日期：

    2026年

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  • Blyssalyn V. Bieber,
  • Faith A. St. Clair,
  • Jesse Quatse,
  • Shane Denecke,
  • Michael Povelones,
  • Megan L. Povelones

摘要

Monoxenous trypanosomatids exclusively parasitize insects and are powerful systems for exploring trypanosomatid biology, the evolution of parasitism, and host–parasite interactions. In addition, they are tractable early-diverging eukaryotes that can serve as model organisms for a variety of conserved processes. We have elaborated on existing methods for culturing the mosquito parasite Crithidia fasciculata and the bumblebee parasite Crithidia bombi, including quantitative assessment of surface attachment and genetic modification. In addition, as cultured strains retain their ability to infect their insect hosts, we describe use of a luciferase-based reporter line to quantitate infection intensities in laboratory mosquitoes.