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This chapter provides a comprehensive set of protocols for preparing diplonemid cells for a range of microscopy applications, including live-cell imaging, fixed-cell fluorescence microscopy, endocytosis assay, immunofluorescence assay (IFA), and fluorescence in situ hybridization (FISH). It presents detailed instructions for slide preparation, cell harvesting, chemical fixation, permeabilization, and fluorescent labelling, with critical steps highlighted throughout. These protocols are designed to be adaptable for different experimental goals, enabling researchers to obtain high-quality imaging data for exploring the cellular biology of diplonemids.

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Preparation of Diplonemid Samples for Microscopy

  • 实验方案

  • 发表日期：

    2026年

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  • Galina Prokopchuk,
  • Daria Tashyreva,
  • Orsola Iorillo,
  • Bungo Akiyoshi,
  • Julius Lukeš,
  • Drahomíra Faktorová

摘要

This chapter provides a comprehensive set of protocols for preparing diplonemid cells for a range of microscopy applications, including live-cell imaging, fixed-cell fluorescence microscopy, endocytosis assay, immunofluorescence assay (IFA), and fluorescence in situ hybridization (FISH). It presents detailed instructions for slide preparation, cell harvesting, chemical fixation, permeabilization, and fluorescent labelling, with critical steps highlighted throughout. These protocols are designed to be adaptable for different experimental goals, enabling researchers to obtain high-quality imaging data for exploring the cellular biology of diplonemids.