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Metabolomics, the comprehensive study of low-molecular-weight metabolites, provides a direct snapshot of an organism’s physiological state and complements genomics and proteomics. Ion mobility (IM) enhanced metabolomics adds crucial gas-phase separation, resolving isobaric and isomeric metabolites for deeper biological insights. Here we describe a method coupling rapid liquid chromatography to IM-enhanced mass spectrometry, which allows high-throughput metabolomics analysis. This method was successfully applied to trypanosomes to elucidate the mode of action of a small-molecule inhibitor in Trypanosoma brucei.

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Metabolomics Analysis of Trypanosomes Using Ion Mobility–Enhanced Mass Spectrometry

  • 实验方案

  • 发表日期：

    2026年

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  • Andrew MacKenzie,
  • Fraser Massie,
  • Tessa Moses

摘要

Metabolomics, the comprehensive study of low-molecular-weight metabolites, provides a direct snapshot of an organism’s physiological state and complements genomics and proteomics. Ion mobility (IM) enhanced metabolomics adds crucial gas-phase separation, resolving isobaric and isomeric metabolites for deeper biological insights. Here we describe a method coupling rapid liquid chromatography to IM-enhanced mass spectrometry, which allows high-throughput metabolomics analysis. This method was successfully applied to trypanosomes to elucidate the mode of action of a small-molecule inhibitor in Trypanosoma brucei.