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Spiroplasma melliferum is a cultivable mollicute that can be used as a proxy model organism to test the inhibitory effects of filtrates, compounds, or substances against phytoplasma. In this protocol, we describe the use of the resazurin-based alamarBlue™ dye for the monitoring of Spiroplasma survival and growth in the presence of presumably inhibitory agents.

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The Use of Spiroplasma melliferum as a Model Organism to Study Potential Antimicrobials Against Phytoplasma

  • 实验方案

  • 发表日期：

    2026年

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  • Amir Fine,
  • Luca Galtarossa,
  • Katrin Janik

摘要

Spiroplasma melliferum is a cultivable mollicute that can be used as a proxy model organism to test the inhibitory effects of filtrates, compounds, or substances against phytoplasma. In this protocol, we describe the use of the resazurin-based alamarBlue™ dye for the monitoring of Spiroplasma survival and growth in the presence of presumably inhibitory agents.