Light-sheet microscopy has emerged as the technique of choice for fast in vivo and ex vivo imaging of biological systems and processes. The selection of the objective lens and camera is a critical decision that dictates the resolution, field of view, working distance, imaging speed, and overall capability of any light-sheet microscopy system. Historically, nearly every light-sheet microscope has selected these two technologies from the life sciences domain. However, there are extremely powerful technologies that have been developed by the adjacent electronics metrology industry. Here, I summarize and discuss the available technologies from this industry and how they can be leveraged to design new, powerful light-sheet microscopy systems.

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A Guide to Leveraging Electronics Metrology Technologies for New Light-Sheet Microscopy Systems

  • Adam Glaser

摘要

Light-sheet microscopy has emerged as the technique of choice for fast in vivo and ex vivo imaging of biological systems and processes. The selection of the objective lens and camera is a critical decision that dictates the resolution, field of view, working distance, imaging speed, and overall capability of any light-sheet microscopy system. Historically, nearly every light-sheet microscope has selected these two technologies from the life sciences domain. However, there are extremely powerful technologies that have been developed by the adjacent electronics metrology industry. Here, I summarize and discuss the available technologies from this industry and how they can be leveraged to design new, powerful light-sheet microscopy systems.