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T cells are key players of the cellular immune system, able to detect and fight pathogens’ invasion. T cells recognize pathogen-derived peptides presented by molecules called Human Leukocyte Antigens (HLA) in humans. The interaction between the T cells and peptide-HLA (pHLA) complexes is driven by the surface T cell receptors (TCRs). This interaction is critical and the first step of T cell activation. The affinity of the TCR for the pHLA is one of the key drivers of T cell activation. In this chapter, we describe the method to determine the affinity of the TCR for the pHLA via surface plasmon resonance.

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Method for Determining the Affinity of the T Cell Receptor for the Peptide-Human Leukocyte Antigen Complex

  • 实验方案

  • 发表日期：

    2026年

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  • Lawton D. Murdolo,
  • Stephanie Gras,
  • Demetra S. M. Chatzileontiadou

摘要

T cells are key players of the cellular immune system, able to detect and fight pathogens’ invasion. T cells recognize pathogen-derived peptides presented by molecules called Human Leukocyte Antigens (HLA) in humans. The interaction between the T cells and peptide-HLA (pHLA) complexes is driven by the surface T cell receptors (TCRs). This interaction is critical and the first step of T cell activation. The affinity of the TCR for the pHLA is one of the key drivers of T cell activation. In this chapter, we describe the method to determine the affinity of the TCR for the pHLA via surface plasmon resonance.