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An important component of the adaptive immune system is the T cells that detect and clear pathogen-infected cells. T cells have surface receptors (TCRs) that recognize pathogen-derived peptides bound by immune proteins called Human Leukocyte Antigens (HLA) in humans. The intricate interaction between the TCR and the peptide-HLA (pHLA) complex drives the T cell activation that, in turn, dictates infection’s outcome. To understand this key interaction, X-ray crystallography has been used to determine the atomic structures of both the TCR and the pHLA after complex formation. In this chapter, we describe the method to form a TCR-pHLA complex, crystallize it and analyze the resulting crystal structure.

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Method for Performing T Cell Receptor-Peptide-Human Leukocyte Antigen Co-complexation and Structural Analysis

  • 实验方案

  • 发表日期：

    2026年

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  • Demetra S. M. Chatzileontiadou,
  • Stephanie Gras

摘要

An important component of the adaptive immune system is the T cells that detect and clear pathogen-infected cells. T cells have surface receptors (TCRs) that recognize pathogen-derived peptides bound by immune proteins called Human Leukocyte Antigens (HLA) in humans. The intricate interaction between the TCR and the peptide-HLA (pHLA) complex drives the T cell activation that, in turn, dictates infection’s outcome. To understand this key interaction, X-ray crystallography has been used to determine the atomic structures of both the TCR and the pHLA after complex formation. In this chapter, we describe the method to form a TCR-pHLA complex, crystallize it and analyze the resulting crystal structure.