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In this chapter, we provide an overview of a fluorescence technique, known as “Protein-Induced Fluorescence Enhancement” (PIFE), to follow the association between a protein and an RNA. Unlike Förster-type resonance energy transfer (FRET), which requires at least two dyes, PIFE utilizes a single fluorophore attached to the RNA, with an unlabeled protein interacting with the nucleic acid. We discuss how both ensemble and single-molecule PIFE studies can be employed to explore molecular interactions and to measure the strength of the interactions in solution with low sample consumption.

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Analysis of Protein–RNA Interactions by Protein-Induced Fluorescence Enhancement (PIFE)

  • 实验方案

  • 发表日期：

    2026年

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  • Joana Weiler,
  • Véronique Arluison

摘要

In this chapter, we provide an overview of a fluorescence technique, known as “Protein-Induced Fluorescence Enhancement” (PIFE), to follow the association between a protein and an RNA. Unlike Förster-type resonance energy transfer (FRET), which requires at least two dyes, PIFE utilizes a single fluorophore attached to the RNA, with an unlabeled protein interacting with the nucleic acid. We discuss how both ensemble and single-molecule PIFE studies can be employed to explore molecular interactions and to measure the strength of the interactions in solution with low sample consumption.