Scholarlink

Circular dichroism is a useful tool to study the impact of mRNA sequence optimization on mRNA structure. mRNA can fold into several conformations according to the sequence of nucleotides. Structural characteristics of mRNA (melting temperature, unfolding trajectories) are important for their biological function. Circular dichroism is a powerful technique to characterize mRNA structure. In this chapter we describe how to analyze protein coding mRNA by Synchrotron Radiation Circular Dichroism (SRCD). The emphasis is put on mRNA synthesis and sample preparation, which are critical for mRNA analysis. We also provide a protocol for the in vivo evaluation of vaccine activity.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Application of Synchrotron Radiation Circular Dichroism for mRNA Structural Analysis

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Ayoub Medjmedj,
  • Josef Hamacek,
  • Frank Wien,
  • Federico Perche

摘要

Circular dichroism is a useful tool to study the impact of mRNA sequence optimization on mRNA structure. mRNA can fold into several conformations according to the sequence of nucleotides. Structural characteristics of mRNA (melting temperature, unfolding trajectories) are important for their biological function. Circular dichroism is a powerful technique to characterize mRNA structure. In this chapter we describe how to analyze protein coding mRNA by Synchrotron Radiation Circular Dichroism (SRCD). The emphasis is put on mRNA synthesis and sample preparation, which are critical for mRNA analysis. We also provide a protocol for the in vivo evaluation of vaccine activity.