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Hepatitis E virus (HEV) is a significant cause of acute hepatitis in humans and an emerging zoonotic disease. HEV genotypes 1 and 2 primarily affect humans, and HEV-3 and HEV-4 are mainly transmitted from zoonotic sources. Transmission occurs through contaminated water, undercooked meat, and, in some cases, via blood transfusions or organ transplants. Globally, HEV is responsible for approximately 20 million infections and 44,000 deaths annually, with the majority of outbreaks in developing countries linked to water contamination. Environmental monitoring, particularly wastewater surveillance, plays a key role in detecting HEV outbreaks. This study outlines a methodology for molecular detection of HEV in water samples, employing ammonium sulfate precipitation for virus concentration, magnetic bead-based nucleic acid extraction, and RT-nested PCR for high-sensitivity detection.

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Molecular Detection of Hepatitis E Virus in Water

  • 章节

  • 发表日期：

    2026年

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  • Juliana Schons Gularte,
  • Meriane Demoliner,
  • Fernando Rosado Spilki

摘要

Hepatitis E virus (HEV) is a significant cause of acute hepatitis in humans and an emerging zoonotic disease. HEV genotypes 1 and 2 primarily affect humans, and HEV-3 and HEV-4 are mainly transmitted from zoonotic sources. Transmission occurs through contaminated water, undercooked meat, and, in some cases, via blood transfusions or organ transplants. Globally, HEV is responsible for approximately 20 million infections and 44,000 deaths annually, with the majority of outbreaks in developing countries linked to water contamination. Environmental monitoring, particularly wastewater surveillance, plays a key role in detecting HEV outbreaks. This study outlines a methodology for molecular detection of HEV in water samples, employing ammonium sulfate precipitation for virus concentration, magnetic bead-based nucleic acid extraction, and RT-nested PCR for high-sensitivity detection.