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Hepatitis E virus (HEV), especially genotypes 3 and 4, is an emerging zoonotic pathogens commonly associated with foodborne transmission, particularly through the consumption of contaminated pork and pork products. This chapter presents a methodology for the molecular detection of HEV in pork meat samples, describing the preparation of raw meat samples for analysis, nucleic acid extraction using magnetic bead technology, cDNA synthesis, RT-nested PCR, and electrophoresis.

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Molecular Detection of Hepatitis E Virus in Pork Meat

  • 章节

  • 发表日期：

    2026年

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  • Meriane Demoliner,
  • Juliana Schons Gularte,
  • Fernando Rosado Spilki

摘要

Hepatitis E virus (HEV), especially genotypes 3 and 4, is an emerging zoonotic pathogens commonly associated with foodborne transmission, particularly through the consumption of contaminated pork and pork products. This chapter presents a methodology for the molecular detection of HEV in pork meat samples, describing the preparation of raw meat samples for analysis, nucleic acid extraction using magnetic bead technology, cDNA synthesis, RT-nested PCR, and electrophoresis.